7 Questions 3 Answers 0 Followers
Questions related from Weidong Huang
Nanoparticle colloid solution has been synthesized by lots of researchers, however, there were few efficient approach to enhance the quantity and purity. Please tell me some suggestions, thank you.
12 December 2018 3,873 0 View
A single fluorescent spot (donor and acceptor were merged as one spot) was obtained as 300 frames through two-channel equipment, the fluorescent intensity of which was analyzed separately. Then I...
04 April 2014 4,293 2 View
The laser power is about 0.2 mW above the coverglass, is that able to obtain clear single molecule fluorescence spots?
04 April 2014 3,149 2 View
I have two complementary oligonucleotides (about 25 nt). How long will they hybridize into dSDNA in a certain buffer at room temperature? Are there some differences between immobilization and...
03 March 2014 1,389 2 View
There were some fluorescence impurities on the coverslip when it was immobilized on the microscope without adding any sample that confused with the real fluorescence spots. The coverslip was...
03 March 2014 9,566 12 View
Now I want to detect fluorescence molecules excited by laser, the power of which is 0.5 mW detected by power meter above the sample. So I want to know whether it is enough to obtain clear images?
03 March 2014 982 4 View
During the process of observing fluorescence, how do I distinguish the real sample from impurity? In addition, how can I identify whether the fluorescence belongs to single molecule or not?
03 March 2014 2,803 3 View
