How to modify Eosin Methylene Blue within limits?

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How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to stain bacterial surface for electron microscopy?

I would like to investigate the surface of bacteria using electron microscopy to assess changes in specific conditions. Is there an established protocol for that? Thank you.

15 July 2024 2,338 2 View

How to assess bacterial permeability?

Is there an established protocol to measure the permeability of bacterial membranes? Thank you

30 June 2024 6,614 3 View

How to immobilize bacteria without morphology alterations?

I have a set of bacteria that I stained with fluorescent dyes to check for viability. The protocol suggests to place 5 ul of the suspension on a slide, cover with a cover glass, and observe on a...

16 June 2024 9,382 1 View

How to transfect environment bacteria?

I would like to transfect bacteria with some plasmids. However, the bacteria are not competent but either isolated from the environment or purchased reference strains. Can I transfect these...

16 June 2024 9,384 6 View

How to describe statistical values in words?

After performing statistical analysis, there is the need to report the p-values in the text. Is there an accepted (published) stratification range? For instance, is 0.04 'slightly' significant?...

28 May 2024 7,724 3 View

How to assess if Kd values (constant of dissociation) are meaningful?

I used Microscale thermophoresis(MST, from Nanotemper) to determine the constant of dissociation (Kd) between a protein (20 nM) and a ligand (1:2 serial dilution from 50 μM to 1.5 nM). I ran the...

04 May 2024 2,066 0 View

How to stain live bacteria for real time tracking?

I would like to track in real time the growth of bacteria using fluorescent miscoscopy. I remember there was at least one plasmid that could be used: transfected bacteria would emit a red or green...

29 April 2024 7,095 3 View

What magnification for organoid imaging?

Hello, I would like to take some publication-quality pictures of organoids. What magnification shall I use? Do I need a 60x or is a 40x enough? Thank you

15 April 2024 8,471 1 View

How to sterilize pipette without autoclave?

Hello, I think I am using a 5 mL pipette that is contaminated with bacteria. Nothing serious, just the usual environmental microbes. The problem is that the tips for the 5 mL pipette are without...

07 April 2024 6,716 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Can a photocatalytic degradation of methylene blue from red mud be pseudo- zero order kinetics?

My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?

29 July 2024 7,404 1 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View

Can we change blood agar in the mCIM and eCIM protocols?

In the modified carbapenem inactivation method (mCIM) and EDTA-carbapenem inactivation method (eCIM) protocols, can we replace blood agar with another culture medium?

10 July 2024 6,352 0 View