When we analyse plant tissues we faced difficulty in modefoed pH for Na-EDTA titration method to determination of Ca and Mg. Notice that the solution was strongly acid due to acid digestion of plant leaves.
I have used z score in one of my research paper. now i want to use some other good statistical methods. Need guidance in this regard. i am working in energy efficient algorithms in cloud computing...
21 January 2021 9,018 3 View
there is a problem with the heating part in soxhelt apparatus, so my question can we use the standard hot plate as a source of heating?
05 December 2020 9,322 2 View
I have alcalase enzyme it's activity 10000U/g and I want to use it in hydrolysis whey protein isolate 90% protein. A suspension of 5% (w/v)WPI prepared so I am asking about the quantity of enzyme...
05 October 2020 9,825 3 View
Hello everyone I conducted sequencing prosedure for RNA genome segment (about 700nt) , for this reason I employed the random hexamer primer for cDNA synthesis . after sequencing (by sanger...
16 August 2020 3,994 3 View
I am an instructer at college of agriculture and I have difficulty to deliver the knowlege to my students in 3-learning especially in applied subjects and topics.
04 June 2020 8,757 14 View
I am currently working on a database and I have tried various methods of machine learning and deep learning, all leads to poor classification/prediction. However, this might show that the two...
27 May 2020 7,894 7 View
As we know some clay minerals and iron oxides have positive charges on Its surface, and viruses have negatively charges, is there any ability to use this feature to reduse or stop the activity of...
22 March 2020 5,007 4 View
I was read in paper in searchable encryption that encrypt bloom filter index in the following way: 1- generate secret key to encrypt bloom filter and query is: the secret key sk (M1, M2, s ),...
03 March 2020 1,448 5 View
I want to implement a specific algorithm for an MD or Monte Carlo Simulation of large molecules. However, I am not familiar with how molecular topology is preserved in packages like AMBER etc. I...
12 February 2020 5,730 3 View
We use the technology of water magnitazation to release inutrients from the soil and leaching salts from soil profile in salt effected soils.
05 February 2020 3,098 3 View
When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...
03 March 2021 4,892 5 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.
28 February 2021 3,640 3 View
Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...
28 February 2021 7,310 3 View
Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...
26 February 2021 5,525 3 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...
23 February 2021 2,049 3 View
I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...
23 February 2021 5,167 3 View
How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?
23 February 2021 3,053 7 View
I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...
22 February 2021 5,324 3 View