The slides are not deparaffinized yet. I'm using a scalpel to scrape off the tumor area from it, however, the area of interest is very small. I had a hard time transferring the scrapping piece into the tube. I saw some youtube videos, they pre-fill the Eppendorf tube with 100% ethanol, then immerse the scalpel into the liquid to ensure all the pieces are collected. Is this a proper way to do it? , if so, how would I get rid of ethanol and proceed with the deparaffinized process?

My next step is to deparaffinized FFPE and extract RNA and DNA with Qiagen kit. Do you think I can pre-fill deparaffinized solution into the tube, and then immerse my scalpel into the solution to collect all the tissue pieces?