Yun Sawa

4 Questions 1 Answers 0 Followers

Questions related from Yun Sawa

No-filter and Aerosol Barrier pipet Tip?

recently I bought the Fisher brand pipette tip:...

30 May 2023 6,104 2 View

How to minimize the lost from scraping FFPW and transfer to eppendorf tube?

The slides are not deparaffinized yet. I'm using a scalpel to scrape off the tumor area from it, however, the area of interest is very small. I had a hard time transferring the scrapping piece...

04 April 2023 4,288 1 View

Do you press your pipette to the second stop to expel all the liquid?

I'm doing PCR and qPCR frequently. My assay is extremely sensitive so I need to pipette a very exact volume from my SOP. From my experience and also my school lab course, I learned to press the...

30 March 2023 982 4 View

Extract DNA and RNA from FFPE tissue, scraping first or deparaffinizing first?

I have unstained FFPE tumor tissue, with the area of interest graphing on the back of the slides. The goal is to scrap off the area of interest, then extract both DNA and RNA. What's the good...

17 March 2023 1,962 1 View