@ Ghaida, spores colour can be seen without the use of a microscope but other features can only be seen with a microscope. A magnification of at least x 400 is recommended for looking at spores. Glass or plastic cover slips (glasses) are placed over the specimen once it is mounted on a microscope slide.

Cood question interested

spores can be determined by wet sieving method using 100 gm soil and dilution method , further observing under microscope.