I need to measure pKa of 3 resins in order to use in bolger formula and calculate delta parameter (acid-base interaction).
For the estimation of carbohydrates there are different methods. As D39 serotypes contain glucuronic acid as one of the monosaccharide sugars, napthoresorcinol can be used to quantify it. if...
17 June 2024 888 0 View
Hi everyone, I aim to isolate MSCs from the bone marrow of the mouse. I put the bone marrow cells into the cell culture flask containing DMEM low glucose+BFGF+vit C. I know that now I have lots of...
03 December 2023 2,612 3 View
Hi everyone, I'm looking for datasets that pinpoint where waste is discharged into the Pacific Ocean, especially around Washington and British Columbia. It's been tough finding info that lines up...
28 November 2023 1,370 0 View
I'm looking for a database which contains the information regarding the name of B2B firms which use AI-based pricing optimization tools. If this database also include any contact information or...
08 June 2023 4,777 1 View
I'm studying on Glaucous-winged gulls, a ground-nesting species. In my study area, there are not many trees, but a lot of bushes. I want to mount some cameras to see their parental behaviour,...
26 April 2023 744 1 View
Hi all, I am fabricating micro-scale arrays of pillars on silicon wafer with SU8-2025; however, after completing the photolithography process and checking the samples with microscope, I found the...
21 March 2023 1,717 2 View
During the cultivation of Chlorella vulgaris in a 500-liter tubular photobioreactor, substantial biomass sedimentation leads to the formation of a biofilm. What steps could be taken to address...
12 February 2023 6,261 2 View
I want to design a filler task for my study as a dummy task so that the respondents in my survey won't understand the intention of the researcher. Does anybody know any article on how to design...
16 December 2022 9,013 0 View
Hi eceryone. We need to create a model. Our dataset contains a bunch of daily values of covariates, including wind direction. But the response variable, which is a count data, is provided at a...
10 October 2022 1,155 6 View
I am trying transformation in S. pneumoniae using Csp1 but somehow it is not working so I am thinking of making electrocompetent cells for the same but I am unable to find a standard protocol for...
26 April 2022 4,756 1 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
24 July 2024 7,293 4 View
The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.
23 July 2024 3,205 0 View
Hello, I am experiencing an issue with mixing micro powder with resin in a 3D printer. The problem is that sedimentation occurs after a short period (about half an hour to an hour). Could you...
23 July 2024 1,672 0 View
https://en.wikipedia.org/wiki/Viscosity
21 July 2024 3,089 4 View
So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...
18 July 2024 4,075 3 View
Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...
16 July 2024 3,692 6 View