Hello, I am trying to look at the efficiency at which a Gene is cut after tamoximen treatment. Thus we got a set of primers that can tell us weather the Gene is cut or not. We wanted to measure out how much of the DNA is cut relative to the the DNA unaffected by the treatment.
Since it is a single PCR primer, could we do some melt curve analysis that would help us determine the relative amounts of PCR products? Or is just adjusting the cycles in regular PCR and running gels the best way to go about it?
Hello
Please explain the "cut" clearer. Are you expecting the deleting a fragment? Where are primer positions from the deletion?
Hello, yes by cut I mean that there will be a fragment deleted from a WT PCR product. While the untreated DNA would be 600bp long after PCR. The band size of the treated DNA is 100bp.
I will look into using a uv spectrometer and see if it would fit our experimental design
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