Cell infection with lentivirus: GFP+ after soring by flow but no protein expression?

I use a plasmid to overexpress 1 protein in 293T and murine BMSC. But I could never succeed. I produced the lentivirus by transfecting the plasmids in HEK293FT cells, together with another 3for...

24 December 2020 6,389 3 View

I keep getting an error for GEOPIV on Matlab?

When doing the geoPIV_RG_run, i keep encountering this error. Error using dataread Trouble reading floating point number from file (row 1, field 2) ==> Dynamic Load Test Free Vibration Error...

20 October 2020 10,004 2 View

Is lentiviral production of antibodies in 293T viable?

Hi, I recently started using lentiviral systems to produce proteins in mammalian cells. Knowing the performance of this impressive system in producing high titers of proteins, I am wondering if...

05 August 2020 5,144 2 View

What solutions do you suggest for molecular field visualization/analysis?

Hello, I wonder what options exist these days for visualizing "molecular field" type data. That is, a 3D scalar field represented by translucent volume rendering or isosurfaces, and overlaid on a...

12 June 2020 861 2 View

How to choose collective variables for ligand binding to protein?

Hello! What is the recommended way to choose collective variables / reaction coordinates to study ligand binding to a protein (e.g., for PMF free energy profiling)? I often see the advice like "Do...

10 March 2020 1,878 4 View

No title

02 November 2019 8,943 3 View

Why an SD of means difference between two groups (e.g. treatment vs. placebo) is equal to a particular SD in each group?

Very frequently in papers, devoted to parallel clinical trials, I face a situation, when a calculated SD for an effect in each group is approximately equal to an SD of effect difference between...

07 July 2019 9,217 4 View

Western Blot - electrophoresis - wavy gel - help!

31 July 2017 6,026 3 View

Quantification problems with imageJ Western Blot?

24 January 2017 7,878 9 View

What aproach we must use to calculate force in electrostatic problems: potential or electric field intensity?

Well known problem of finding potential for metal sphere and point charge, solving with "mirror image" trick give you 2 different answers for the force, acting on point charge. One when we use...

14 December 2016 9,365 5 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

How to validate regridding results of environemental data to higher spatial resolution in R?

I am working with several continous, environmental marine datasets at varying original spatial resolutions. My aim is to regrid each dataset to one common, high resolution grid. I am currently...

22 February 2021 2,290 2 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View

Assistance in finalizing and publishing an article on human resource management in forestry?

Dear colleagues! We have prepared an article on the current status of human resources potential of municipal state forest management institutions of Kazakhstan. Since this is my first experience...

16 February 2021 8,611 3 View

Pile-Raft connection?

Greetings, I was wondering what is the optimal way to connect the reinforcement at the top of the pile (the part that connects to the raft foundation): -The pile bars are left straight and...

16 February 2021 2,007 3 View