How to measure inter-rater reliability for multiple responses (check box) on same question?

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How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

How to convert all .csv file to grayscale image and then save them?

I have a database of experiment .csv files. how to extract the feature from this .CSV file with max-min normalization? I need a matrix(with values between 0 to 255) to be converted to a grayscale...

04 February 2021 7,773 3 View

How to rectify the miscomputation of Pressure and Continuity in HVAC CFD Simulation Using OpenFOAM?

I'm using OpenFOAM to run a CFD study of thermal comfort within a hall. I'm using OpenFOAM v1912 and BouyantBoussinesq(Simple&Pimple)Foam solver. eI have 6 inlets and outlets each with specific...

21 December 2020 1,303 3 View

Is there any other microbes (except microalgae) that could efficiently remove nitrogen and phosphorus content from wastewater ?

I was studying the biological treatment (anaerobic) of wastewater biomass. I found that anaerobic bacteria take a comparatively longer time to biodegrade nitrogenous compounds. Is there any other...

28 October 2020 921 3 View

Would you suggest me a formula for calculating energy yield from biohydrogen and biomethane production?

I was looking for a formula to calculated the obtained energy yield via biohydrogen and biomethane production using anaerobic digestion process. The unit is in SI unit (x) mLH2/g VS and (y) mL...

20 October 2020 8,668 5 View

Is my retinal GFAP staining accurate?

I am doing immunofluorescence staining on retinal sections from RCS rats to assess GFAP. As shown in my attached image, the GFAP staining is not consistent with most literature I've seen with...

05 October 2020 8,105 3 View

How to optimize reaction conditions to achieve maximum lanthanide chelate attachment of the surface a polymer encapsulated nanoparticle?

Hi, The aim of this project is to attach a lanthanide chelate onto the surface of a polymeric nanoparticle that is encapsulated with polystyrene maleic anhydride (the anhydride hydrolysis in...

29 September 2020 7,386 2 View

Is it possible to do image processing using Arduino/NodeMCU? If so, which camera module would be better?

We have already completed a project where we have done image processing using raspberry pi. Now we want to do the project using Arduino / NodeMCU.

28 September 2020 1,486 3 View

What Important factors should I keep in mind while writing a review paper?

I am trying to write my first review paper. I don't have much knowledge about writing it. If you could provide me with some valuable points, I would be very thankful.

15 September 2020 10,025 10 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View