Is it possible,PFAS analysis in LC-MS/MS?

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How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Handheld Portable Raman spectroscopy systems - recommendations and experience?

Hi all, I am looking for some general feedback and information from those that have experience using handheld/portable systems? I am looking at the prospects of using an off the shelf system from...

07 February 2021 9,691 3 View

Why can't I run sample more than 40 mins in Agilent HPLC even after loading method for 45 mins?

Hi, I am using an Agilent 1200 series and Chemstation software. When I try to set run-time more than 40 mins (for ex. 45 mins or more than that), run-time is getting over within 40 mins even after...

26 January 2021 7,136 3 View

2.6 Drying gas flow controller cannot regulate the requested setting after a fixed amount of time?

We keep getting this error on our Agilent 6470 TQ. We were told it could either be our N2 generator or filter traps. We've called the manufacturer of our generator and they believe it's working...

21 January 2021 2,969 1 View

Where can I buy Agilent software?

Hello. I have old agilent instrument, 6340 with 1100series HPLC, LC-Ion trap MS. We have software for LC, chemstation, but, not for MS. I asked Agilent korea if I can buy some software, and they...

05 January 2021 1,970 3 View

How to get rid of tailing peaks?

I have been making a method to determine chlorhexidine using an LC-MSMS (ESI+ modus). My solutions are made in acetonitril/water (65/35) and 0,2% formic acid is added to ionize the compound. My...

14 December 2020 3,855 6 View

Why is the gene expression of one sample uniformly higher than that of the other in the microarray gene expression analysis?

Hi all, Currently, I'm working on a project to analyze gene expression from RNA which is extractred two different samples by microarray. The results showed that almost all gene expression was...

12 December 2020 3,918 2 View

What may be the reasons for broad spectrum at 4.8 ppm in proton NMR (taken in DMSO-d6) for Pd(II) complexes?

The synthesized Pd(II) complexes involved -NH group attached to C=S moiety. The 1H proton NMR taken in DMSO-d6 solvent consisted of a very broad peak at 4.8 ppm with an integration of 6. They are...

09 December 2020 9,960 3 View

What is the reason vacuum performance degradation of the AAS analyser?

I'm working with Agilent 200 series atomic absorption spectroscopy in the lab. There is no problem when I first started the analysis. As time passes, for example half an hour after starting the...

01 December 2020 3,867 3 View

What are typical Cu sources causing Cu adducts in ESI MS?

Hi everybody, currently I observe quite frequently Cu-adducts on my ESI high resolution MS, especially when analyzing ligands. There was no Cu used for the synthesis, so it must come from the...

21 October 2020 5,630 5 View

I'm having difficulties with RT shifts of 0.25 min (15 sec) in a urine sample?

I performed 10 repeat injections and separations of a single urine sample on a reverse phase HPLC-DAD system. Everything was kept constant (Flow rate 1ml/min, column temp 35C, injection volume...

14 October 2020 1,173 3 View

Bruce Neagle

Yes. See attached:

https://pages.waters.com/2023-04-PFAS-Appnotebook.html?xcid=ad-soc_27812?utm_source=linkedin&utm_medium=singleimage&utm_campaign=2023global_pfas_campaign_q2&utm_content=message1&utm_term=

William Letter

This is a very common and popular application. Please try a keyword search on the web using a popular search engine (e.g. GOOGLE, BING) to find EXAMPLE application notes, white papers, articles etc. Also search the instrument manufacturer's own application note directory for related examples. Note: Any example settings used in the articles may not apply to YOUR instrument. Their are no "exact" settings as they must be determined EXPERIMENTALLY, for each method, on each instrument, for each sample (this is known as 'Method Development') so please contact an experienced local chromatographer with experience using your detector and software for assistance.