How can I measure in vitro hepatoprotective activity of a phytoprodct (extract, pure compounds) in a lvier cell line (HepG2)/ what are the suitable agents that can be used to induce cell injury (cytotoxic agents)?
Alok Nahata
For inducing liver toxicity, you can use ethanol, paracetamol and carbon tetra-chloride as hepatotoxins and silymarin as a standard hepatoprotective.
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Harita Parikh
You can assess cytotoxicity, measure leakage of enzymes, ros generation, apoptosis, cell cycle analysis, etc...
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