How to measure Carbon-13 enrichment using GC-MS?

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Michael Kohlstedt

Dear Naga,

first, you have to convert your mass isotopomer distribution (MID) data in a summed fractional labeling (SFL) as described here:

Article Metabolic Network Analysis of Bacillus clausii on Minimal an...

Chapter Metabolic Activity Profiling by 13C Tracer Experiments and M...

Make sure that you correct your GC-MS data for natural isotope abundance prior to calculation:

Article Correcting mass isotopomer distributions for naturally occur...

Finally, you can calculate the 13C enrichment in % depending on the 13C enrichment of the glucose tracer used (see paper above).

Regards

Michael

Eleuterio Umpierrez

Take into account that not all GC/MS fulfill the specification for these type of analysis. The best HRMS, but some normal MS has enough resolution and discimination if the enrichment it is higher.

Regards,

Eleuterio

Isam Eldin Hussein Elgailani

Please look at the following below links which may help you in your analysis:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5192448/pdf/metabolites-06-00042.pdf

https://orbilu.uni.lu/bitstream/10993/34841/1/metabolites-08-00015.pdf

Thanks

Naga Veera Yerra

Thank you very much!!