Naga Veera Yerra

17 Questions 22 Answers 0 Followers

Questions related from Naga Veera Yerra

How effective is Q-Exactive for top-down proteomics?

We are planning to use an q-exactive mass spectrometer for top-down proteomics . There is an HCD collision cell in mass spectrometry. How well does q-exactive's top-down proteomics work?

19 October 2023 8,442 1 View

How media enrichment is differ from intracellular enrichment?

I noticed that media enrichment is different from intracellular enrichment from Glucose 13C tracer studies of TCA cycle intermediates. How do i co-relate both enrichment.

16 November 2022 6,551 0 View

How mixture of carbons coming from Glucose to Glutamate in TCA cycle?

I noticed 13% -1Corbon, 27% -2 Carbons, 15%-3Carbons, 10%-4Carbons and 4%-5Carbons coming from Glucose to Glutamate in the TCA cycle noted by isotope tracer studies. Please explain the mechanism...

10 November 2022 833 1 View

Software for untargeted metabolomics?

Dear folks, I am going to work on untargeted metabolomics with Agilent QToF. Please suggest to me the best software for untargeted metabolomics. Thank you!!!!

08 January 2022 8,067 6 View

Why good crystal formation give good MALDI spectra?

In MALDI analysis good crystal formation of Matrix and Analyte gives quality of spectra. Why quality of results depends on crystal formation?

01 January 1970 8,131 3 View

Light chain quantification by using Mass spectrometry?

Please suggest best way of intact light chain absolute quantification by using Mass spectrometry.

01 January 1970 3,501 5 View

How to measure Carbon-13 enrichment using GC-MS?

Dear folks, I am working on C13 labeled glucose TCA cycle. Please explain how to calculate M+1, M+2, M+3, M+4…….. enrichment. Thank you!

01 January 1970 9,971 4 View

Samples collected in EDTA tubes will result in low zinc level but our results are increased why?

Samples collected in EDTA tubes will result in low zinc level but our results are surprisingly increased. Please explain what could be the reason.

01 January 1970 8,856 3 View

Difference between Thermo biopharma finder and Proteome discoverer software?

Please explain the difference between Thermo biopharma finder and Proteome discoverer software uses. Thanks!

01 January 1970 5,043 2 View

IgG extraction from human serum for mass spectrometry analysis.

I am looking for less cost igG extraction method from human serum for mass spectrometry applications. i used Melon Gel IgG Spin Purification Kit but it is very expensive. Please suggest me any...

01 January 1970 1,820 12 View

What is the number of proteins identified by lc-ms?

Hi, what is the maximum number of serum proteins that can be identified by using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) without nano liquid chromatography (nano LC)....

01 January 1970 4,374 1 View

Identify signature protein by using mass spectrometry

what is the best method to extract proteins from serum and tissue sample? what is the best method to identify the signature protein/peptide between serum and tissue samples by using mass spectrometry?

01 January 1970 3,733 2 View

How to choose the most suitable standard lipid for our specific Mass spectrometry application?

I am looking suitable standard lipid for quantifying phosphatidylethanolamine (PE) from serum samples. As you may be aware, standard lipids, such as those derived from different sources (Soy, egg,...

01 January 1970 7,534 2 View

How to isolate light chain from reduced igG?

I am currently working on a project that requires the isolation of light chain from reduced IgG for bottam up proteomics-Mass spectrometry. Kindly provide insights or recommendations. Thanks!

01 January 1970 3,730 4 View

How to analyse haparin in lc/ms?

Is it possible to obtain the exact molecular weight information after digesting haparin (10–30 kDa)? or what data the LC-MS digested haparin analysis can provide? Thanks!

01 January 1970 3,480 0 View

How to calculate Decision limit and Detection capability

How to calculate Decision limit (CCα) and Detection capability (CCβ) from LOD LOQ data. is there any free software?

01 January 1970 8,835 4 View

Why am I detecting signals from multiple compounds in MRM even though Q1 ions are different but Q3 ions are the same, with different retention times?

I am performing an LC-MS/MS analysis in MRM (Multiple Reaction Monitoring) mode. I have three different compounds. Each compound has a different Q1 (precursor ion) but produces the same Q3...

01 January 1970 6,291 8 View