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Questions related from Naga Veera Yerra
We are planning to use an q-exactive mass spectrometer for top-down proteomics . There is an HCD collision cell in mass spectrometry. How well does q-exactive's top-down proteomics work?
19 October 2023 8,321 1 View
I noticed that media enrichment is different from intracellular enrichment from Glucose 13C tracer studies of TCA cycle intermediates. How do i co-relate both enrichment.
16 November 2022 6,463 0 View
I noticed 13% -1Corbon, 27% -2 Carbons, 15%-3Carbons, 10%-4Carbons and 4%-5Carbons coming from Glucose to Glutamate in the TCA cycle noted by isotope tracer studies. Please explain the mechanism...
10 November 2022 752 1 View
Dear folks, I am going to work on untargeted metabolomics with Agilent QToF. Please suggest to me the best software for untargeted metabolomics. Thank you!!!!
08 January 2022 7,987 6 View
In MALDI analysis good crystal formation of Matrix and Analyte gives quality of spectra. Why quality of results depends on crystal formation?
01 January 1970 8,049 3 View
Please suggest best way of intact light chain absolute quantification by using Mass spectrometry.
01 January 1970 3,427 5 View
Dear folks, I am working on C13 labeled glucose TCA cycle. Please explain how to calculate M+1, M+2, M+3, M+4…….. enrichment. Thank you!
01 January 1970 9,886 4 View
Samples collected in EDTA tubes will result in low zinc level but our results are surprisingly increased. Please explain what could be the reason.
01 January 1970 8,795 3 View
Please explain the difference between Thermo biopharma finder and Proteome discoverer software uses. Thanks!
01 January 1970 4,979 2 View
I am looking for less cost igG extraction method from human serum for mass spectrometry applications. i used Melon Gel IgG Spin Purification Kit but it is very expensive. Please suggest me any...
01 January 1970 1,757 12 View
Hi, what is the maximum number of serum proteins that can be identified by using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) without nano liquid chromatography (nano LC)....
01 January 1970 4,310 1 View
what is the best method to extract proteins from serum and tissue sample? what is the best method to identify the signature protein/peptide between serum and tissue samples by using mass spectrometry?
01 January 1970 3,655 2 View
I am looking suitable standard lipid for quantifying phosphatidylethanolamine (PE) from serum samples. As you may be aware, standard lipids, such as those derived from different sources (Soy, egg,...
01 January 1970 7,475 2 View
I am currently working on a project that requires the isolation of light chain from reduced IgG for bottam up proteomics-Mass spectrometry. Kindly provide insights or recommendations. Thanks!
01 January 1970 3,661 4 View
Is it possible to obtain the exact molecular weight information after digesting haparin (10–30 kDa)? or what data the LC-MS digested haparin analysis can provide? Thanks!
01 January 1970 3,409 0 View
How to calculate Decision limit (CCα) and Detection capability (CCβ) from LOD LOQ data. is there any free software?
01 January 1970 8,763 4 View
