Could someone provide the cif file of classic organic salt/organic cocrystal TTF-TCNQ?

27 September 2020 178 3 View

How to avoid particle aggregation when using Laser Direct Infrared (LDIR) to analyze microplastics?

I am trying to use LDIR system to identify microplastics of different types. However, significant aggregation of microplastics was observed during analysis. Especially for particles with a...

17 September 2020 1,532 1 View

Is P value reliable in a smaller data set when p<0.05 in a larger data set?

I have two data sets, one size is 123, I called it A, and the other size is 28, I called it B. and I also have a variable that I think it has an association with clinical outcome. I observe the...

07 July 2020 390 9 View

Seeking a fiber/particle reinforced metal-based composite with superior fatigue resistance..?

Hi, I'm seeking a fiber/particle reinforced metal-based composite with superior fatigue resistance, which should be of high tensile strength and young's modulus and low production cost. In the...

23 December 2019 3,639 1 View

Is it possible to set charge value of different atoms in a first principles calculation?

I want to introduce two charged atom in a supercell using first principles calculation (such as ABINIT, VASP). Is it possible? If not, why?

13 February 2019 118 4 View

How to solve CST mesh problem when discrete port is rotated?

I'm building a transmitter, receiver pair and comparing the difference when I rotate the pair of antennas while keeping distance the same. When the discrete port is rotated by 60 degrees along...

28 January 2019 4,168 2 View

Modelling cloth in COMSOL?

Hi there, I have a problem modelling a piece of cloth in COMSOL. The key feature of cloth is that it has no bending stiffness or out-of-plane shear stiffness. Could you tell me how to set the...

21 January 2019 2,264 2 View

Is it possible and beneficial to learn probability distribution of input data when we apply machine learning methods like artificial neural network?

Hello, everyone! In recent time I am learning an online course called "learning from data". The lecturer showed the below learning diagram you can find from this website:...

29 October 2018 2,510 3 View

Annoying random deletions in mutagenesis experiment. How to mix the problem?

We use iproof polymerase from Bio-Rad and a pair of outward primers to PCR the whole plasmid, then phosphorylate the ends, ligate the PCR product into circular plasmids, and then transform. It...

23 January 2018 2,503 1 View

Some question about beta zeolite

23 May 2017 3,468 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

How could a transcriptional factor purified as inclusion bodies (by using urea) exhibit a ratio 260/280>1?

I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...

05 February 2021 2,530 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View