There are many methods to achieve ECM materials for bioprinting, but which method is the best to preserve growth factors within tissues?
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
When I was reading the paper, I came across this passage, "Around 2000, international investment arbitral tribunals began to pay attention to the public interest issues involved in such cases, and...
08 May 2024 8,492 0 View
Wie baut man Kugellager in Modelica? Es dient der Modellierung, Simulation und Visualisierung seines dynamischen Verhaltens. Wie soll das Verhältnis zwischen den verschiedenen Komponenten des...
23 April 2024 8,420 0 View
I am doing research on 3D-printed skin substitutes, however, I don't see any related articles reporting this. Any suggestions for reading? Thanks
07 January 2024 4,146 0 View
I’m currently conducting research on telomeres in THP-1 cells. During my gel electrophoresis experiments using a 1% gel, I’ve encountered an issue where the DNA seems to be stuck at the top of the...
29 October 2023 9,561 7 View
Hello everyone, I've been working on producing a hydrogel from the extracellular matrix (ECM) derived from the human placental amniotic membrane. I've followed a process where I first...
11 October 2023 8,721 0 View
I saw the effects of sleepiness in the meditation group in the results section. The conclusion drawn by the author in the abstract is: 【follow‑up analyses showed that sleepiness uniquely moderated...
21 September 2023 7,092 1 View
I am reaching out for advice on some challenges we have faced with inducing pluripotent stem cells (iPSCs) to differentiate into mesenchymal stem cells (MSCs). Plan 1: We choose P30 generation...
31 August 2023 1,327 0 View
I have attempted various transfection reagents including Lipo3000/Lipo2000/Hieff Trans™ Liposomal Transfection Reagent, both GAPDH positive control and custom siRNA targeting gene of interest at...
20 August 2023 6,146 2 View
I am exploring parenting styles (four styles) as predictors with father, mother, and adolescents' perspectives on adolescent well-being (two outcomes) with two mediators (one with four subscales...
16 July 2023 601 0 View
need to review articles on care giving in dementia and challenges faced
12 August 2024 6,424 1 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
What can you look for to know that the circuit model being used is overfitting the data. How do you know when overfitting is occurring?
31 July 2024 7,425 0 View
I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
30 July 2024 1,412 8 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
Hi, I will run an experiment where reaction time will be the dependent variable, tested in 2 conditions (with the same participants). I want to control for a confounding variable (individual...
20 July 2024 1,943 1 View
One of the papers I read by Toby, where (title of the paper was "R factors in Rietveld analysis: How good is good enough?"), he tells us that to get good chi square value, you must have low Rwp,...
17 July 2024 9,668 4 View
I'm currently working on the implementation of pulses (legumes) in bakery applications. In one case, pulse flours could be added to bread to increase the nutritional value. Pulses (in particular...
17 July 2024 7,293 3 View
Hello, I have a problem in classification of "Additive Manufacturing" techniques. There are several classification for Additive Manufacturing e.g. Powder, Extrusion, Resin etc. Actually, I am OK...
15 July 2024 139 3 View