How to maximumly maintain grwoth factors when decelluarization during the process of ECM preparation?

08 January 2024 0 5K Report

There are many methods to achieve ECM materials for bioprinting, but which method is the best to preserve growth factors within tissues?

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How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

Are there scholars who have conducted statistical analyses on international investment arbitration cases ?

When I was reading the paper, I came across this passage, "Around 2000, international investment arbitral tribunals began to pay attention to the public interest issues involved in such cases, and...

08 May 2024 8,492 0 View

Visualisierung von Kugellagern in Modelica？Wie baut man Kugellager in Modelica?

Wie baut man Kugellager in Modelica? Es dient der Modellierung, Simulation und Visualisierung seines dynamischen Verhaltens. Wie soll das Verhältnis zwischen den verschiedenen Komponenten des...

23 April 2024 8,420 0 View

Are there any optimized strut and pore size for bioprinted skin?

I am doing research on 3D-printed skin substitutes, however, I don't see any related articles reporting this. Any suggestions for reading? Thanks

07 January 2024 4,146 0 View

What cause my DNA stuck at the top of my well and why are there 2 lines in between my smear??

I’m currently conducting research on telomeres in THP-1 cells. During my gel electrophoresis experiments using a 1% gel, I’ve encountered an issue where the DNA seems to be stuck at the top of the...

29 October 2023 9,561 7 View

Unable to Form Hydrogel from Decellularized Amniotic Membrane ECM: What Might I Be Missing?

Hello everyone, I've been working on producing a hydrogel from the extracellular matrix (ECM) derived from the human placental amniotic membrane. I've followed a process where I first...

11 October 2023 8,721 0 View

Struggled with the conclusion of this article?

I saw the effects of sleepiness in the meditation group in the results section. The conclusion drawn by the author in the abstract is: 【follow‑up analyses showed that sleepiness uniquely moderated...

21 September 2023 7,092 1 View

:Issues with induced differentiation of iPSCs into MSCs - Any suggestions?

I am reaching out for advice on some challenges we have faced with inducing pluripotent stem cells (iPSCs) to differentiate into mesenchymal stem cells (MSCs). Plan 1: We choose P30 generation...

31 August 2023 1,327 0 View

How to efficiently transfect siRNA into Jurkat T cells?

I have attempted various transfection reagents including Lipo3000/Lipo2000/Hieff Trans™ Liposomal Transfection Reagent, both GAPDH positive control and custom siRNA targeting gene of interest at...

20 August 2023 6,146 2 View

How to run dual mediation models using structural equation model with multiple perspectives?

I am exploring parenting styles (four styles) as predictors with father, mother, and adolescents' perspectives on adolescent well-being (two outcomes) with two mediators (one with four subscales...

16 July 2023 601 0 View

any study on protective factors of care givers of dementia and resilience ?

need to review articles on care giving in dementia and challenges faced

12 August 2024 6,424 1 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

What are some signs that an ECM is overfitting the Nyquist plot?

What can you look for to know that the circuit model being used is overfitting the data. How do you know when overfitting is occurring?

31 July 2024 7,425 0 View

How to we see transcription activity of the bhlh79 family is at the N-terminal or C-terminal?

I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...

30 July 2024 1,412 8 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

ANCOVA for between-subject design?

Hi, I will run an experiment where reaction time will be the dependent variable, tested in 2 conditions (with the same participants). I want to control for a confounding variable (individual...

20 July 2024 1,943 1 View

How to decide whether the refinement is correct or not, based on Rwp and Rexp factors by Fullprof?

One of the papers I read by Toby, where (title of the paper was "R factors in Rietveld analysis: How good is good enough?"), he tells us that to get good chi square value, you must have low Rwp,...

17 July 2024 9,668 4 View

Does the bread-making process lead to a sufficient reduction in lectin activity of bean flour to guarantee food safety?

I'm currently working on the implementation of pulses (legumes) in bakery applications. In one case, pulse flours could be added to bread to increase the nutritional value. Pulses (in particular...

17 July 2024 7,293 3 View

How to classify 3D Bioprinting as a part of Additive Manufacturing?

Hello, I have a problem in classification of "Additive Manufacturing" techniques. There are several classification for Additive Manufacturing e.g. Powder, Extrusion, Resin etc. Actually, I am OK...

15 July 2024 139 3 View