Dear all,
I have a dataset from several lakes describing their zooplanktonic communities (species abundances) and I would like to use multivariate methods to analyze the data (I am provisionally oriented to a RDA / CCA).
My question is: how to manage repeated samples? I sampled each lake several times (more specifically from 1 to > 30 times). I am not very familiar with multivariate analyses, but I feel that this unbalanced sampling could cause some problems (something similar to pseudoreplications?!). Can anybody help me? Is it sufficient to include the variable "lake" in the explanatory matrix of the environmental factors?
Thank you
Rocco