By soil washing process...you can get the procedure online

Ok thank you Drlatief Ahmad

Hai,

You can do Zn deficient soils by different ways

1. Collect Zn deficient soils based on already done soil test values / soil maps if available

2. Using Zn free nutrient solution without soil -Yoshida or any other solution

3. Using Silica - Zn free material as growing media and we can test desired levels of Zn in that media (Zn deficient and Zn sufficiency levels if you are screening genotypes)

Thank you Surendran U. For Silica can we use sand? I need to study plant upto fruiting stage. So perlite can not be the good option.

You can use the sandy or alkaline soils to then add other nutrient fertilizers except Zn.

Use of soils with Zn deficiency (already soil test is necessary).

If SIlica means it will be devoid of any other nutrients, so that your results for with Zinc and Without Zinc - will be good. However if you prefer to use sandy soil, then do the soil test for Zn and accordingly you plan the experiment

yes

Thank you..

The best way is to select a soil which is Zn deficient. if you will follow certain other procedures other than growing plants, you are likely to change other properties of the soil.

Since it is a lab experiment, what you have got to do is to bring a sandy soil, wash it several times to expel all nutrients and then prepare a solution contains all nutrients except Zinc. Apply this solution to your pots whenever needed.