How to determine the number of iterations of A-optimal projection?

I have read the article "A-Optimal Projection for Image Representation" (A-Optimal Projection for Image Representation). The authors used an iterative method to solve the optimization problem,...

06 September 2020 3,257 1 View

How to randomly seperate a region into subregions with different area?

Dear all, I have a watershed and wish to randomly split the watershed into different 'artificial' farms, and the farm area should follow an exponential distribution as found empirically from...

20 January 2020 3,782 3 View

Anyone could explain if lentivirus is pathogen?

Because lentiviral vectors are derived from the HIV-1 pathogen, biosafety issues are an important concern. I was asked to indicate whether if lentivirus is animal or human pathogen when I try to...

10 July 2019 2,950 3 View

How can I get the lysate from human/mouse red blood cells to do a western-blot ?

How can I get the lysate from human/mouse red blood cells (RBC) to do a western-blot ? My interest gene has been widely reported to express on RBC and not well established in other tissues. And...

25 March 2019 6,972 3 View

Whether gap junction will effect neuronal capacitance measurement ?

i use whole cell patch to measure neuronal tau and resistant. with the equation tau=RC, Capacitance is calculated. I wonder if neuron has coupled with other neurons via gapjunction, the measured...

24 February 2019 7,702 7 View

How to collect data about transnational agricultural industrialization?

My research has encountered some difficulties, and I feel that the number of cross-border literature I read is not enough, especially in the field of agricultural economy to study the literature...

01 November 2018 9,461 3 View

Anybody would like to edit a special issue "Fitness Landscapes as revealed by in vitro selection"?

Hello everyone, Recently, I would like to establish a special issue "Fitness Landscapes as revealed by in vitro selection" in Life (http://www.mdpi.com/journal/life). If you are interested in...

22 May 2018 5,691 3 View

Representative HUMAN Breast Cancer, Lung Cancer, Melanoma Cell lines?

I am planning to do a very preliminary experiment using a few representative solid cancer cell lines. Probably Three for each cancer types. For Breast cancer, I am thinking to use MDA-231, MCF7...

03 May 2018 4,230 4 View

How to dilute solid milk from stomach?

We are trying to detect oxytocin from milk in the rat stomach. We found out that milk there are solid like beancurd. any suggested method to dilute them before centrifuge? Many thanks for help!

23 April 2018 777 1 View

Are there any special requirements on protein in RNA-protein UV-crosslinking assay ?

20 February 2018 8,060 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View