How can I obtain the expansion orbital coeffiecients in Hartree Fock program?

23 December 2020 6,756 3 View

Have you cloned a PCR product containing 2X2A sequences into a vector that contains another 2A sequence?

Hi! I am having problems cloning a 1.6 kb fragment containing 2 different genes and 2X2A peptides into a vector that I already used to clone other fragments (digested with PmeI/BamHI). The vector...

17 December 2020 5,218 3 View

How to validate CNVs (deletions and duplications) in human samples by qPCR?

Hello, I am interested in the validation of CNVs (deletions and duplications) in human samples by qPCR. We are trying to validate the primers for the experiments, but the primers are not working...

21 October 2020 5,896 2 View

Can I create a CitNet Explorer (VOS viewer) map using more than one "cvs" file or do I have to compile then?

Can I create a CitNet Explorer (VOS viewer) map using more than one "cvs" file or do I have to compile then?

02 March 2019 614 1 View

What kind of body measures can I take in wasps to contrast differences between individuals from different nests?

27 January 2019 7,520 11 View

What kind of body measures can I take in wasps to contrast differences between individuals from different nests?

31 December 2018 7,497 12 View

Any literature that explores the relation between environmental education or capacity building and community participation?

30 November 2018 2,506 7 View

How can I remove a monolayer of 4-Mpy?

Hi, I have functionalized arrays of Au nanostructures with a monolayer of 4-Mercaptopyridine (4-Mpy). However, I would like to use again the same substrate for other measurements. I tried to...

29 November 2018 8,678 2 View

How can I remove a monolayer of 4-Mpy?

Hi, I have functionalized arrays of Au nanostructures with a monolayer of 4-Mercaptopyridine (4-Mpy). However, I would like to use again the same substrate for other measurements. I tried to...

10 November 2018 671 2 View

What parameters or variables could affect the performance of a microalgae culture?

The production of microalgae depends on several physical or chemical parameters: light, CO2, O2, temperature, pH. These can affect only at a certain stage of the crop. What other parameters...

09 October 2018 8,781 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View