Dear Sai,
Il have a same answer. But u can also use ethanol instead of butanol for preparing solution. I prepare without acetic acid than also the charring pattern was good.
Dear Sai,
There are a few different versions of the recipe for ninhydrin stain solution but most include a little acetic acid, such as this one:
0.2 g Ninhydrin
0.5 ml Acetic acid
100 ml n-Butanol
4.5 ml Water
(taken from curlyarrow.blogspot.co.uk)
I've also included a link to a useful page which shows staining of amino acids on example TLC plates, where you can see the Rf and colour for each amino acid. Hope this helps,
Mark
http://www.reachdevices.com/TLC_aminoacids.html
Respected Ana Reis and Mark Thompson,
Thanks A Lot for the answers. They were very helpful.
Dear Sai,
Il have a same answer. But u can also use ethanol instead of butanol for preparing solution. I prepare without acetic acid than also the charring pattern was good.
8 % (w/v) Ninhydrin solution- 8 g of ninhydrin dissolved in 100ml acetone and keep on cold condition for future use.
What is the role of acetic acid? Is it necessary to add to the solution?
Optimum/maximum colour development with ninhydrin reagent is in the pH range of 4 to 6; that is why acetic acid is added.
Dissolve 4 g of ninhydrin in 100 ml ethanol and mix well without acetic acid
It works better if you prepare a 0.2%(w/v). Add 0.2 g of ninhydrin in 100 ml of ethonol. You can dissolve thoroughly using ultrasound.
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