How to model in HFSS or FEKO Free Space Material Characterization?

I am having trouble setting up this problem in HFSS or FEKO. In HFSS, I've tried both FEM and FEBI-IE solvers. With FEKO, I've tried it's MoM solver and default settings. I am would like model...

09 February 2021 375 2 View

Statistical Test for Single Group over Multiple Conditions & Different Time Lengths?

I have a dataset with an unusual configuration and was hoping for guidance on choosing a method to test for changes in mean. For context, this is operational data tracking vehicle arrivals at 10...

20 January 2021 4,429 4 View

How long does MQAE solution last once prepared?

I am wanting to prepare a small quantity of MQAE solution. Once prepared, how soon should it be used? How fast will it degrade? Is there a way I can store a leftover amount for future use? Are...

20 December 2020 1,416 3 View

Why do many stock buffers ask you to adjust pH and then add more water?

Could someone please explain why many stock buffers ask you to adjust the solution's pH and subsequently increase the volume to a set amount? For example: Add 500g of powder X to 800mL of...

09 December 2020 1,882 3 View

How to estimate sample size when you think the effect size will be reduced (compared to previous studies)?

Hi, I am planning a follow-up experiment to a previous study and have a question about estimating sample size: For the sake of simplicity, the previous study showed that Condition 1 had more...

19 November 2020 7,948 6 View

What is angular velocity of projection of uniform circular motion?

12 November 2020 481 5 View

Peptide solubility in DMF (n,n-dimethylforamamide)?

I am looking to dissolve a suite of "acidic" peptides for, ultimately, a validated LCMS assay. Using DMF as an initial solvent (20% of final volume) but some peptides precipitate when aqueous is...

15 October 2020 9,171 1 View

How do crush foraminifera for d18O analysis?

I am looking to combine and crush 5 foraminifera to create a homogeneous carbonate powder that I will then be sending out for oxygen analysis. Does anyone have any good methods to make these...

14 October 2020 8,128 7 View

Troubleshooting co-IP from a small amount of brain tissue?

I am looking to perform a co-IP; however, I am using a very small amount of tissue to begin with from region specific microdissection in mice (2-3 300um sections per animal). I am concerned that...

07 October 2020 8,937 3 View

Can Stimulus Packages safeguard businesses during the Covid-19 Pandemic?

Research Proposals

02 October 2020 6,404 9 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View