My protein is forming soluble aggregates, i have tried upto 300mM NaCl, 2mM of beta-mercaptoethanol, or DTT, with 10% glycerol, 35 mM Tris-cl (pH-7.4), and 1mM PMSF. PI of my protein is 5.6 . My protein has a flexible N-terminal alpha-helix. I am using E.coli BL21 DA3 strain for expression. i am using 0.25mM IPTG for induction. Please let me know if you have any suggestion. it would be a great help

Thanks in advance

Deepak

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