How to make a protein soluble, which is forming soluble aggregates?

My protein is forming soluble aggregates, i have tried upto 300mM NaCl, 2mM of beta-mercaptoethanol, or DTT, with 10% glycerol, 35 mM Tris-cl (pH-7.4), and 1mM PMSF. PI of my protein is 5.6 . My...

02 June 2023 6,370 4 View