How to make a job's lot of graphene oxide and a ligand using Fluorescence ?

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Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

How to design a boost converter for a mppt controller?

I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...

28 February 2021 3,637 2 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

What low-cut filter (high Pass frequency) to be used for response spectrum (Tripartite plots) ?

What low-cut filter (high Pass frequency) to be used for the response spectrum (Tripartite plots)? I am currently working on differentiating between hard/soft site responses in Indo-Gangetic...

21 February 2021 9,273 2 View

How to calculate refractive index (n and kvalue) against energy (in eV) data for alloyed nanomaterials through Independent Phase Approximation method?

Suggest me the method or some other software to calculate the above said data for nanoparticles.

21 February 2021 3,350 7 View

What is the right way to conjugate two acids?

How to conjugate two acids with amine linker?

18 February 2021 4,083 2 View

How to find the air side pressure drop in a spiral fin tube bundle cross flow heat exchanger?

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18 February 2021 1,109 2 View

Any methods to train a neural network with random (stochastic) and non-measurable variable?

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17 February 2021 1,909 3 View

Solid Phase Extraction of drug product containing nanoparticles ?

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16 February 2021 1,389 2 View

What is best technique to quantify necrotic cell death of specific cell populations?

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08 February 2021 733 6 View

Does the pressure(-600 mmHg) affects pH?

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03 March 2021 4,892 5 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

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27 February 2021 4,703 1 View

How does charge on working electrode changes with rising potential?

During performing CV of a GCE or modified GCE, we rise the potential in the first half of the cycle and reduce it in reverse half. During the first half, what is the nature of charge on GCE or...

25 February 2021 7,994 3 View

How to delaminate copper in water-based anodes?

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24 February 2021 3,785 3 View

Thermal reduction of graphene oxide.?

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23 February 2021 4,155 4 View

What kind of steel moulds are used for hot pressing metal powders?

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22 February 2021 2,965 4 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

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22 February 2021 5,324 3 View

Color of spiro-ometad film?

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21 February 2021 1,831 4 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

Asma Iqbal

To synthesize graphene oxide (GO) and a fluorescent ligand:

Graphene Oxide (GO) Preparation: Exfoliate graphite using Hummer's method, oxidizing to GO. Sonicate to disperse into stable aqueous suspension.

Fluorescent Ligand Synthesis: Modify ligand (e.g., dye or quantum dot) with functional groups (e.g., amine, carboxyl) for GO interaction. Employ bioconjugation or covalent linking.

Ligand Attachment: Incubate GO and fluorescent ligand in solvent, allowing conjugation via functional groups. Utilize EDC/NHS coupling or click chemistry for stable attachment.

Purification: Centrifuge to separate unbound ligand, wash, and resuspend in desired buffer. Analyze using fluorescence spectroscopy for ligand binding confirmation.

Fluorescence Characterization: Measure fluorescence emission and excitation spectra, assessing ligand interaction with GO. Observe shifts, intensity changes, or FRET (Förster resonance energy transfer) effects.

Ayushi Mishra

Asma Iqbal nicely copied from chatgpt. being a researcher please provide authenticated answers don't just copy-paste and spread misinformation.