We are using KAPPA kit for direct DNA amplification which is giving good results for the desired amplification. In the case of RNA isolation from thrip, we are using Quigen kit, which is also giving good results. I want to amplify RNA virus from thrip. Can anyone suggest a kit for the direct amplification of RNA strand from the thrip just like the KAPPA kit?
I am not sure about the the KAPPA kit but you can use via reverse transcription-quantitative polymerase chain reaction (RT-qPCR) as reported in the attached paper. This will detect and quantify you virus
Article Detection of LTR Retrotransposons Reactivation induced by in...
There are many commercial kit to do what you wont. I recommend SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase.
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