Can anyone help with a problem regarding intergenic region amplification?

01 January 2014 1 5K Report

We are trying to amplify intergenic region of Tospoviruses, but getting difficulty in PCR amplification. I want to know whether it is because of loops present in that region or any other specific problem.

If loops are present in the sequences and interfering in the amplification then how to get rid of them any specific protocol, any papers any sort of suggestions are most welcome.

Badges
Science topic

More Ashish Anil Ingle's questions See All

Which is the best insecteside/ pesticide used to control thrips population in USA and in European countries?

We want to apply the same to study the impact of such insecteside on Thrips population in India.

01 February 2016 8,416 1 View

How to make a direct amplification of RNA strand?

We are using KAPPA kit for direct DNA amplification which is giving good results for the desired amplification. In the case of RNA isolation from thrip, we are using Quigen kit, which is also...

31 December 2013 3,941 3 View

How can I isolate DNA from a single Thrips? Can anyone suggest to me protocols for this?

I am trying to isolate DNA from Thrips. DNA isolation kits are not for single DNA thrips isolation.

07 August 2013 7,765 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View