How to remove the salt and get purified DNA in Silica Magnetic bead-based Nucleic acid extraction?

I am doing nucleic acid extraction (manual silica magnetic bead-based) from the serum sample. After the extraction, I have taken the OD 260/280 in UV spec. There is no protein contamination. But...

04 November 2020 1,061 2 View

Could you tell me the composition of the wash buffer in RNA extraction (Silica Magnetic Bead-based) and its proportions?

I am doing silica magnetic beads-based RNA isolation. I am facing some impurity issues because of washing. So kindly share some best washing buffer composition of wash buffer for especially for...

01 November 2020 6,536 2 View

Best book for Fluid Structure Interaction?

Practical methods along with the basic behind Fluid Structure Interaction.

11 June 2020 9,743 9 View

Most of the Research papers are found more theoretical and previous work but only few paragraph spent in experiment and result , Why ?

My research is going on Traffic analysis , Prediction and Intelligent Transport System. I have good concept in theory but I found that most of the papers are not elaborated experiments setup...

17 April 2020 407 12 View

Can I use Phosphate Buffer for Antibody and Fluorescence conjugation?

I am going to use Phosphate Buffer (pH 6.5) is an alternative of MES buffer (pH 6.5) for Antibody and Fluorescent Microspheres conjugation procedure. The literature I am referring to mentions MES...

22 December 2019 794 3 View

Do I need to do a polymerization reaction under a nitrogen atmosphere?

I am polymerizing styrene with Potassium Peroxodi Sulfate and Acrylic acid by an emulsion polymerization method. The literature I am referring to mentions stirring the reagents with a nitrogen...

18 December 2019 8,158 4 View

IoT Challenges with Healthcare?

In want do some work in the security of IoT with Healthcare , please suggest me some area , in which area our scholar can do some work. If possible provide some detail from 2018 and 2019 Dr....

12 July 2019 6,628 3 View

Performance Analysis of Big Data ?

How performance of the Big data can be analysis, which framework should used ? Is Hadoop and Spark Framework are good or other tool should use , is any Source for code or algorithm can find?

01 July 2019 348 3 View

I want to study about antibody antigen binding for chikunguniya and malaria virus? Where can i find relevant material?

Study for Virus diagonesis

23 April 2019 9,210 5 View

Address of Tanusri Mandal is wrong . How to rectify?

Tanusrei Mandal, an author of the paper, entitled "Calcite solubilization by Bacteria: An Novel method of Environmental pollution control ". Published in Geomicrobiology Journal Vol.32, PP.846...

23 January 2018 1,566 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Plant research: Watering uniformity/consistency. How important is it?

Trying to gather insight from an unresolved discussion with a colleague here, and either the available literature surrounding this topic and/or my keywords in search of are lacking. Context:...

21 February 2021 6,389 3 View

No title

The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

Is there a way to grow biofilm in lab to mimic biofilms in showers and baths? Also, how can I measure it?

Hello, I am new to biofilm research and have a question about growing and measuring biofilms. We want to research the effect of a type of enzymes in breaking down biofilms in showers, baths, hot...

14 February 2021 3,522 3 View

Can downdraft gasifier for RDF be scaled up?

Dear researchers, In literature, so far I am seeing that downdraft is usually used for small-scale purposes (10kW-10MW). One of the reasons behind is "they do not allow for uniform distribution...

08 February 2021 5,403 1 View

(abaqus) Questions about the uniform stress condition of spherical particles ??

I want to simulate a uniform stress condition in the vertical direction on the surface of a spherical particle. Since it is very difficult to describe the boundary conditions of a complete...

31 January 2021 2,726 1 View

Are there any technical and safety tips or details for hot salt baths?

29 January 2021 6,036 2 View

Abaqus, how can I add a composite layup to a complex shape?

I am designing a knee simulation focusing on the meniscus. I want to add different material layers to the meniscus. I have tried to add composite layup to the menisci in a uniform way but the...

27 January 2021 4,998 1 View

What should be the current and voltage for nickel coating of around 15 micron for steel specimen and also the bath composition?

I need the complete setup information.

21 January 2021 6,455 4 View