I have HCT116 cells with unknown passage number.I'im culturing them in complete RPMI 1640 media. when I first thaw the cells in t25 flask (with 20%FBS media), they attach the flask and grow. when they are at 80% confluency I passage them onto t75 flask (10% FBS media). but when I switch to t75 they start to die. what are the possible reasons?