Müzeyyen Çoban

3 Questions 1 Answers 0 Followers

Questions related from Müzeyyen Çoban

Why DMSO did not kill my cells?

I'm performing an MTS assay (PROMEGA CellTiter 96® AQueous One Solution Cell Proliferation Assay) with healthy and carcinoma cells. I apply %10 DMSO for positive control to my treatments. I...

27 December 2024 1,827 1 View

How to maintain HCT166 cells?

I have HCT116 cells with unknown passage number.I'im culturing them in complete RPMI 1640 media. when I first thaw the cells in t25 flask (with 20%FBS media), they attach the flask and grow. when...

08 October 2024 3,737 0 View

What could be the reason of that I could not observe any signal on my PVDF membrane?

Hi, I performed western blotting for the 8 times. In two of them I used wet-transfer and the rest of them assayed with semi-dry transfer. I checked my buffers, systems, gels and everything. I used...

08 September 2024 2,086 2 View