How to link an enzyme to nanoparticle?

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Rafael de Oliveira Pedro

Dear rajendra kumar reddy Gajjala

I would say it will depend on the enzyme. For example, Glucose Oxidase remains active after binding on the gold surface.

I would suggest reading the following reference, it might be useful:

Article Enzyme Immobilization on Nanoporous Gold: A Review

I hope this has helped.

Kind regards.

Shamsher S. Kanwar

Many methods are available for loading enzymes on nanoparticles. Use surface activation of nanoparticles by TEOS and then bind/ load the enzyme. Most enzymes remain functional in this approach.