Amorphous XRD Data?

Dear colleagues, I analyzed my material using XRD analysis and the material has shown amorphous behavior , how can I specify the composition of the material is there any extra analysis I can...

24 December 2020 1,280 10 View

Is it possible that a IncX and IncF plasmid fused?

I recently performed plasmid extraction and nanopore sequencing. I have found what looks like a plasmid with replicon types IncX and IncF. Even with spAdes, canu, unicycler (illumina and nanopore...

29 September 2020 7,778 2 View

Any suggestions for a simple method to reduce the viscosity of bacterial growth media?

Hi, I am working with a bacteria from the genus Paenibacillus. I am having trouble separating it from the media (PDB), and difficulties in extraction of secondary metabolites from the media,...

04 August 2020 9,974 3 View

Can you put cells from -80 to liquid nitrogen?

Hi all, I have kept cells in -80 for several months, can I transfer them to liquid nitrogen now after all this time? I am afraid they would not survive.. I appreciate your input Thanks!

12 July 2020 3,612 2 View

Solar cell exciton binding energy ?

30 April 2020 1,620 12 View

Can I make a primerset (for RT-qPCR) for one single transcript variant? If yes, how?

I am trying to perform a RT-qPCR and I would like to design a primerset for one single transcript variant for RT-qPCR. The problem is that it is very difficult to get primers that only bind on one...

22 April 2020 3,071 4 View

Is there a comprehensive list of qualitative research methodologies to be found?

I am searching for a (relatively) comprehensive taxonomy or list of qualitative research methodologies. A list that goes beyond Creswell's "five approaches" (likely not exhaustive, but nonetheless...

24 November 2019 9,989 8 View

How would you suggest that an author be transparent about ideas that came up only during the review process, and by a reviewer rather than the author?

I am conducting a qualitative study. The manuscript was returned to me from the initial review with the recommendation that I revise and resubmit. So far so good. One of the reviewers, however,...

23 September 2019 7,963 8 View

Too many bands after digestion with restriction enzymes?

Hello Everybody, I am in the process of extracting a natural plasmid containing the CTX-M 1 gene. I do not know what plasmid (type/Inc etc) is present in my sample but I have been successful in...

28 May 2019 832 3 View

How can you prevent cells from clumping on a coverslip of a 24 well plate?

Hi, I'm trying to stain my cells after transient transfecting them but each time I get the cells either over confluent, or clumped in the middle even though I swirl the dish in an 8 shape to...

15 March 2019 6,585 2 View

How to figure out easily the right journal with the right scope for your paper?

According to my experience most of the selected journals during paper submission are based on journal text matching suggested results from Springer, Elsevier,,,Etc. then the manuscript happened to...

01 March 2021 1,786 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

Why am I getting 'Infinity' and 'NaN' in my mixed-mode energy release values?

Hi everyone, I am currently running a mixed-mode simulation of a DCB using a user-defined cohesive zone model (UEL), however, the results I am getting don't make sense. I used the same UEL for...

24 February 2021 4,157 1 View

What does it require to be ready for e-Commerce in developing countries?

Most developing countries are not ready for e-Commerce? What dimensions and indicators are there to measure e-commerce readiness of a nation? Please share framework or model and evaluation...

22 February 2021 8,132 6 View

Why is my CFD problem (mini heat sink rectangular channel) not converging while having good quality mesh?

My CFD problem is having two interconnected rectangular channel, which is known as mini heat sink. I have already performed parallel flow inside of an interconnected mini heat sink. However, I...

19 February 2021 3,209 3 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

For PC-3 cell line. How long before irradiation do I have to synchronize my cells to G0/G1?

I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...

09 February 2021 9,480 1 View

Up to what extent website personalization can help in creating urge to buy impulsively?

Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...

09 February 2021 4,430 6 View

Grid connected inverter using virtual synchronous machine?

Hi everybody, currently, I am working on the grid-connected inverter. I want to make my grid-connected PV system a PLL-less system. I have studied some reachers are implementing a virtual...

07 February 2021 6,078 2 View

How to eradicate the deteriorated contact resistance in the electrical or electronic devices, like SCR or contactor' pin cable ?

Through years operation, some of the devices' pins connection gradually become worse and finally burn out due to the high temperature at the connection point. We can check out these risk points...

04 February 2021 7,401 5 View