I would like to filter bacteria solution using a certain designated membrane and count the different in concentration of bacteria before and after. Hence, it is preferred to have the bacteria dead so that the factor of bacteria multiplication can be ignored. Does anybody know a method to this?
Hi Muhammad,
if you plan to count consantration with OD measurements, I would suggest to use RNA protect bacteria, Qiagen. It stops bacterial growth,but you can not do CFU count on the plate after adding it because the bacteria stabilizes...
And very small amount would work....
good luck...
the easiest way to kill the bacteria is to alter the ph of the culture or by subjecting the culture to 70% alcohol sometimes even repeated centrifugation at low RPM can kill bacteria.I think any one of these methods would be helpful.
Dear Mohammed Syukri:-
Bacteria are single-cell microorganisms that exist everywhere and reproduce quickly. Some kinds of bacteria are harmless, while other types cause disease and sickness. These types are called pathogens. If not controlled, harmful bacteria can take over the home, causing a family to be plagued with health problems. People can kill bacteria in the home through several methods including sterilization, pasteurization, cold temperatures, antiseptics, and disinfectants.
Sterilization uses intense heat to kill bacteria, and pasteurization uses mild heat to lessen the number of bacteria in foods. Cold temperatures combat bacteria as well. When food is refrigerated or frozen, it is preserved and protected from bacteria. Antiseptics are applied to human skin to eliminate bacteria that may collect on an open cut. Finally, a disinfectant can be used to clean toilets, sinks, and floors.
People can kill bacteria by washing their hands after using the bathroom, touching raw food, changing a baby's diaper, cleaning up after a pet, or taking out the trash. It's important to use soap and warm water, scrubbing the hands, washing under the fingernails, and including the wrists in the hand-washing process. Wash for at least 15 seconds in order to kill bacteria on the skin.
Dear Mr Al-Ouqaili
I do not agree with you when you say "Wash for at least 15 seconds in order to kill bacteria on the skin" because the action of soap on the skin does'nt kill the bacteria, it will only slide on the epidermis and then will be take away by the water.
Regards,
Yanis Guenoune
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