I have to isolate phages from sea water samples obtained from nuclear power plant to study the thermal ecology aspects of phage infection to their host.
How to isolate viruses/phages from seawater samples
In case of isolation phages from seawater samples using tangential flow filtration (TFF) either alone or in combination with ultracentrifugation and then recover specific viruses using the soft agar overlay method. The amplification of the associated virus(es). Virus and host were separated using conventional centrifugation and filtration and virus‐host systems are identified by culturing techniques. You may found more information in this following article.