We're curently working with the HT29-MTX-E12 cell line clone. We chose this clone for its mucus production. However, this generally desirable feature can be difficult, e.g. for immunocytochemical staining. I'm sure I read in a paper that a group detached the mucus (enzymatically?) before proceeding with their staining protocol, but I cannot find it anymore.
Would anybody here have an idea or a suggestion?
Thanks a lot in advance.
Angela Kaempfer
Well, in case anybody comes across this with the same question: Article The Interaction of Helicobacter pylori with the Adherent Muc...
N-acetyl cysteine is the answer.
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