How to isolate good quality RNA for sequencing purpose from grey mangrove (Avicennia marina) plant, any idea about suitable method with protocol ?

01 January 2020 1 7K Report

Various methods/ protocols are available for RNA isolation from plant, from commercially available kits, TRIzol to CTAB but major problem while dealing with RNA is degradation, we tried RNA isolation from plant plant Avicennia marina by TRIzol method four to five times but every time we got degraded RNA, that will not work in sequencing, Anyone please suggest us what would be modifications in currently using TRIzol method or have to move for different method please suggest...

Asemoloye Micheal Dare

For me I use ctab, marcaptoethanol and licl2 protocol.. It works good fir rna isolation

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