How can I prevent Ngn2 neuron-astrocyte co-cultures from peel off the coverslip?

Hello everyone, I've been co-culturing hiPSC-derived Ngn2 neurons with human fetal astrocytes, and at the time of grabbing the coverslip from the plate (24 wp) the cells peel off the coverslip. I...

03 September 2020 2,947 1 View

Any suitable DARPP-32(Thr34) antibody?

Does anyone know about any supplier for DARPP-32(Thr34) antibodies that work well in immunofluorescence? Specifically to prove in mouse and rat tissue.

01 February 2020 4,049 1 View

Does anyone knows why output parameter is not working in this PyQgis script?

The problem must be the definition of the output string, because I tried the same code but writing a route directly on the parameters list and it worked. This is the code I wrote: import...

07 August 2019 6,108 4 View

Problem confirming discriminant validity (SmartPLS). Could you help me?

14 September 2016 4,923 9 View

Does UV-crosslink also work between proteins? And how is it possible to "reverse" it?

UV-crosslink is widely used to bind together proteins and RNA for co-isolation. Does it works also to connect proteins with other proteins? Is it possible to "reverse" such crosslink? For example,...

27 June 2016 7,546 3 View

How to reorder factors on plotMeans using R commander?

I did a plotMeans to visualize the results of Kruskal-Wallis test between groups. But I need to reorder the levels of a factor because the default order is alphabetical and I want to show it in...

19 January 2016 949 3 View

How to reorder factors on plotMeans using R commander?

I did a plotMeans to visualize the results of Kruskal-Wallis test between groups. But I need to reorder the levels of a factor because the default order is alphabetical and I want to show it in...

31 December 2015 404 3 View

Why you have gone EC3 page "Emerging Sources Citation Index"?

10 November 2015 5,928 1 View

Activated charcoal to remove pigments during DNA extraction

Has anyone ever used activated charcoal to remove pigments during DNA extraction? In which form should it be added? Should it be sterilized? Is efficiency dependent on temperature, pH and time?...

06 July 2012 4,191 8 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View