How can I prevent Ngn2 neuron-astrocyte co-cultures from peel off the coverslip?

Hello everyone, I've been co-culturing hiPSC-derived Ngn2 neurons with human fetal astrocytes, and at the time of grabbing the coverslip from the plate (24 wp) the cells peel off the coverslip. I...

03 September 2020 2,947 1 View

Does anyone knows why output parameter is not working in this PyQgis script?

The problem must be the definition of the output string, because I tried the same code but writing a route directly on the parameters list and it worked. This is the code I wrote: import...

07 August 2019 6,108 4 View

Problem confirming discriminant validity (SmartPLS). Could you help me?

14 September 2016 4,923 9 View

Does UV-crosslink also work between proteins? And how is it possible to "reverse" it?

UV-crosslink is widely used to bind together proteins and RNA for co-isolation. Does it works also to connect proteins with other proteins? Is it possible to "reverse" such crosslink? For example,...

27 June 2016 7,546 3 View

How to reorder factors on plotMeans using R commander?

I did a plotMeans to visualize the results of Kruskal-Wallis test between groups. But I need to reorder the levels of a factor because the default order is alphabetical and I want to show it in...

19 January 2016 949 3 View

How to reorder factors on plotMeans using R commander?

I did a plotMeans to visualize the results of Kruskal-Wallis test between groups. But I need to reorder the levels of a factor because the default order is alphabetical and I want to show it in...

31 December 2015 404 3 View

Why you have gone EC3 page "Emerging Sources Citation Index"?

10 November 2015 5,928 1 View

How to interpret vs. test in MCA analysis run by FactoMineR in R commander?

I did a MCA analysis using FactoMineR. I know how to interpret cos2, contributions and coordinates, but I don't know how values of v.test should be interpreted. Thank you

09 October 2015 8,383 3 View

Activated charcoal to remove pigments during DNA extraction

Has anyone ever used activated charcoal to remove pigments during DNA extraction? In which form should it be added? Should it be sterilized? Is efficiency dependent on temperature, pH and time?...

06 July 2012 4,191 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View