The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

17 February 2021 3,062 4 View

Natural frequency extraction with ABAQUS?

I have modeled the RC beam with simply supported BC and I have got the frequency value for the case of the beam, but I have 150 modes for each analysis and I have to see them one by one to find...

05 January 2021 8,626 3 View

How can i figure out whether nanoparticles in my synthesis crosslinked or not?

i prepare protein nanoparticles. in one of my synthesis i use trifle amount of crosslinker and the nanoparticles stick in the bottom of plate. how can i understand that nanoparticles become...

03 December 2020 559 8 View

How to examine the substitution of ligands bound to protein during the MD simulation?

I have simulated a complex ( A protein docked with two ligands), and the ligands are bind to two different protein sites. Still, from the start of the simulation, ligands are positioned in other...

27 November 2020 7,848 4 View

How to change the atom numbers of a residue in a pdb file?

I am trying to dock 2 proteins in HADDOCK server, but it repeatedly shows the following Error for the second pdb file: " Error in PDB file. Your PDB contains multiple residues with number 1 in...

25 November 2020 4,753 1 View

Is it possible that formazan Crystal formation took more than four hours ?

Hello dear scientists: When I treated the Pc12 cell line with H2O2, after 2h the cells seemed to be dead. I didn't observe crystals, so I didn't add DMSO and left it with mtt in the...

20 October 2020 1,106 3 View

How can i detecte the fluorescence and non-fluorescence complex?

hello kind regards I study the structure and activity of the digestive enzymes. The enzyme I studied contains 5 amino acids tryptophan. In one of the studies, I study the effects of a food dye on...

04 October 2020 8,563 3 View

How to increase the number of nanoparticles in fe-sem?

I produce a protein nanoparticle complex,but in fe-sem, I see a few number of nanoparticles,maybe 2 or 3 ,however the color of suspension solution is quite milky and this is a sign of...

29 August 2020 8,812 5 View

Why would a negative control have apoptosis in the Annexin V-PI Flow Cytometry?

06 August 2020 3,627 22 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

A good day to you, Great minds. How do I evaporate hexane and Ethyl acetate extract of bacterial metabolite?

I'm really struggling with it. It takes hours on a water bath at 50 degrees

03 February 2021 1,619 3 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Why some samples don't give any peaks in HPLC when the rest does?

Hello everyone, Last week we ran an HPLC assay with bacterial supernatant samples. The metabolites measured were SCFA and I used a dilution factor of 2 for some samples and 7 for others (because...

22 January 2021 8,667 6 View

What is the role of pO2 and pCO2 in cell culture?

Using a Nova bioprofile (https://www.fannin.eu/wp-content/uploads/2018/07/BioProfile-Flex.pdf) to sample my CHO culture daily I get various metabolite results and also pO2 and pCO2 results. I am...

13 December 2020 7,998 1 View

How to identify possible metabolite from chromatogram?

We have done the analysis for a pre-clinical study and we have observed additional peaks in the chromatograms. We think that these peaks are metabolites. The concentration of the additional peaks...

02 December 2020 6,134 10 View

What is an efficient way of extracting metabolites for fungal isolate from soil for test on its antimicrobial activity?

Hi, I'm finding a method to extract metabolites to evaluate its antimicrobial activity but the identification of the fungi is not done yet. I'm looking for a universal way to extract the...

24 November 2020 2,273 3 View

Pseudomonas that lose their fluorescence?

I am working on Pseudomonas fluorescent strain identification, I found that some of isolate that I have been conserve at 80c last year have loosing their fluorscent appearance on new culture....

09 November 2020 9,181 4 View