Dear Sir, please suggest me for inoculation and tumor development in mice using EAC cells in 3 groups of 3 mice intraperitoneally at different viable cell concentration i.e. 1X106 , 1.5X106 and 3X106 respectively. But even after a month, there is no sign of liquid tumor. Please suggest me for the best way to develop mice tumor model with EAC cells.
EAC xenografts are considered to be syngeneic models, and there are some that you could use to compare your procedure with (see more info here: http://altogenlabs.com/xenograft-models/syngeneic-models/). EAC cells came from mice, so it should be sufficiently simple to make them grow in host mice (especially immunodeficient ones). I would use matrigel if you want to make a solid tumor, and follow any previous protocols you can find on the topic.
- Badges
- Science topic