We have a Flask that contains broth, and we want to inoculate it with Bacteria inoculum, Can we simply take a touch by the loop or by micropipette?
You could do that but it does depend upon the flask size and volume. If a small flask with 5-10 ml volume you can inoculate directly with a loop, but for a large flask it is hard to get a sterile loop deep inside the flask in a sterile manner. Normally you would use the loop to inoculate a culture tube with media, let that grow, and then use some volume of that to inoculate a larger flask. I generally like 1:100 to 1:500 dilutions to be sure you have many generations in log phase, but it does depend upon what the intended use is.
It depends on the goals of your research.
If you want to simply grow a pure culture on a liquid medium after having grown it on an agar medium, it is perfectly possible to transfer the colony to a small amount of medium.
If the goal is biomass accumulation, then you may need more material, for example, you can wash off the agar slant or pre-grow the culture in a small amount of biomass and then transfer to the flask with more broth.
If you are doing any calculations or determining the relationship between biomass/metabolite accumulation, then more precision may be needed, for example, pre-determining the concentration of the applied cell suspension and then transferring the exact amount of suspension to the broth using a micropipette or glass pipette.
Dear Ali Safaa
Depends on the type of search and its goals, you can use both loop, or micropipette but i prefer micropipette
Ali Safaa can you please tell me the recommended amount of inoculant required to inoculate maize seeds ?
Haseeb Ahmad
Maize inoculants are mainly applied over seeds before sowing, which is an additional practice that presents risks of lowering the germination rate if the seeds are mechanically damaged during this process.
https://www.frontiersin.org/articles/10.3389/fmicb.2017.01873/full
Dump the seed into your seed drill, broadcast spreader, or whatever type of planter you are using. Then apply the appropriate amount of inoculant to the seed. Stir the mixture until you feel that the inoculant has come into contact with the majority of seed. Then plant the seed as normal.
https://mbsseed.com/what-is-inoculant-and-do-you-need-it/#:~:text=Dump%20the%20seed%20into%20your,plant%20the%20seed%20as%20normal.
Article Accessing inoculation methods of maize and wheat with Azospi...
Haseeb Ahmad
Thats what i found on a quick search, you can also ask the best Prof. of Agriculture and Agronomy, (Rk Naresh) his Scientific Knowledge is really High, and many other researchers that are specialized in this field, wish you all the success
https://www.researchgate.net/profile/Rk-Naresh-2
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