I have collected several host-specific ecto-mycorrhizal samples from the field and I am willing to inoculate them (Spore suspension) into the same host seedlings to observe growth variation against controlled ones. I am having difficulties comprehending how to inoculate them? I have prepared spore suspension using distilled water dissolving some portion of the fruiting body (crushed). and stored them in refrigerator for later usage. Is my process of preparation good to go or there are more better ways?
Thanks In Advance
Hello again, new friend Md. Abdur Rahman! Ectomycorrhizal fungi mostly spread with the growth of mycelium in the soil, so a spore suspension would not be the most natural way of inoculating. You could inoculate sterile wheat bran or sterile beet seed, incubate until the substrate is well colonized, and sprinkle that inoculum around the plant you want to infect.
Nina Shishkoff should the inoculation of sterile wheat bran or sterile beet seed be done with spore suspension?
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