02 February 2016 17 8K Report

We are injecting collagenase NB8 to the pancrease,

17 minutes in 37 bath,

Washing twice,

Filter the suspension through a 425um diameter wire mesh

Use Histopaque to seperate the cells 

and wash twice.

Do self sedimentation 6 times..

and yield only 300 islets.

How can I improve the method?

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