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Questions related from Yael Shushlav
I find different volumes and different number of cells to inject Wistar rats. I want to inject the maximal volume in order to have a good-size tumor. I injected 200ul by accident and the rat died...
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All articles describing this model give 5% dextrose in drinking water without food. Can I give them hi calorie drink during their healing period (2 days before surgery and until 4-5 days after it)?
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And where can I purchase NC/Nga mice? I want to evaluate a compound similar to Tacrolimus and I need the most relevant model to investigate TH2 interleukins.
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We are injecting collagenase NB8 to the pancrease, 17 minutes in 37 bath, Washing twice, Filter the suspension through a 425um diameter wire mesh Use Histopaque to seperate the cells and wash...
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