Dear all, is there any one here working with bile acids? I want to inject Taurolithocholic 3 sulfat (TLCS) at have high concentration with limited volume. As instructed, the solibidity of
TLCS is only 1mg/ml in PBS at pH 7.2. I wish to have 4 times higher concentration. Thank you for your kind help!
Sincerely,
Trung
Hi Trung,
We usually dissolve our bile acids (including sulfated ones) in methanol or DMSO depending on the type of experiments. Of course, you need to make sure DMSO/methanol to not interfere with what you are studying.
For some bile acids that are harder to dissolve, you can also try sonicating for a couple minutes. That helps!
If methanol does not interfere with your assay, I'd recommend you to dissolve TLCS in methanol. We have dissolved up to 6mg/mL of TLCS in Methanol
Good luck with your experiment!
Solenne
Hi Solenne, thank you very much for kindly sharing.
The idea of sonification is really nice!!
I inject IV invivo, so I could not use DMO or methanol. I used DMSO for isolated cells and it work well with < 1% DMSO.
I tried in PBS, and supprisingly, the solibility of TLCS in PBS is 5 time higher than information written by the company. I injected it safely till now :)