Bacterial cultures are prepared in bulk quantities for reducing the COD level of various industrial effluents in tap water mixed with 0.1 % of peptone, Nutrient broth for nutrient purpose and yellowish appearance. 0.3% of starch is added to gain the viscosity. Inoculation is about 0.001% of bacteria. Cultures are supplied in 50 liters of closed can containers.
But the issue is that, the shelf life is not more than 2 months.
What can be done to increase shelf life of culture?
We have observed shelf life of consortium of bacterial cultures for as many 8 months at room temperature. But I agree with you , shelf life microbial cultures in liquid broth is a serious issue , regardless of whether or not applied in mass scale or field scale.Nowdays bacterial capsules are used for such bulk uses.
Dear Anoop Sir,
Thank you for your valuable response. Shelf life here also includes the desirable property of culture to degrade pollutants.
As you mentioned life of 8 months. Do we have some specific formulation for it?
Difficult to reveal since our patent is under consideration..
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