Hello, I

am using lactate glo (luminance) assay to measure lactic acid in extracellular medium. The cells I am using don’t have a high glycolytic activity and tend to prefer oxidative phosphorylation for energy. When I use mitochondrial toxins such as Rotenone, I see an increase in lactic acid production. But, my assay window is small (1.5 – 2 fold) compared to my vehicle treated samples.

How can I increase my assay window? I have tried longer incubation time with compounds, as well as different glucose concentration. I am thinking about using a different cell line such as HepG2 cells.

Your suggestions are highly appreciated it. Thank you.