We try to express one protein in p.pichia, this protein could be detected in the medium when the yeast growed in 100ml flask but it disappeared in the 1L flask. What is the reason?
Hi Haixia,
was the yeast at the same growth phase in both flasks?
1. Keep and check the pH.
2. Add Methanol induction every 12h instead of every 24h.
3. Keep high aeration.
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