We are trying to isolate RNA from hard corals, however we are running into a few difficulties if anyone has advice.
We have tried a couple of protocols.. one where we grind the skeleton and tissue together and use a kit. While our samples have high yields of RNA present, they show a lot impurities on the bioanalyzer. We also tried another protocol, where we remove the tissue first from the skeleton, but our final pellets contained a lot of salt. We tried an additional washing step but it improved only a little with very low yield. Does anyone have any recommendations?
Thank you!
This is a sample type I've never worked with, but based on the specific problems you're describing I might be able to help. Could you post the bioanalyzer traces that show contamination? Also, for your other method, how did you determine that there was excessive salt? Washing pellets with ethanol is an adequate but inefficient way to remove salt, could you run it over a column instead?
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