How to improve low A260/A230 ratio in RNA samples isolated from whole blood by QIAmp blood RNA mini kit from Qiagen?

18 January 2024 0 10K Report

I am using Qiagen Blood RNA Mini kit to isolate RNA from whole blood.. and the A260/A230 is quite low, even below 1. My main downstream application is RNA-seq. and I am not sure if this low ratio would affect the sequencing.

is low A260/A230 due to low concentration of RNA 20-50 ng/μL), presence of EDTA in the blood samples, or buffer carryover? how can I improve this ratio?

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