Why can I not detect other targets than GAPDH?

Hello, we're trying to detect Keratin 14 and 15 in Keratinocytes as well as FN-1 and CD90/THY1 in Fibroblasts from human skin. As reference gene GAPDH and Vinculin are used. In our first...

07 January 2021 6,652 3 View

Does anyone know instruments to measure "mental health literacy" in teachers?

Hi there, I am looking for questionnaires measuring mental health literacy in teachers and educators. does anyone know any studies in this area?

16 September 2020 1,682 4 View

Which solution do you use for low level actinide decontamination of laboratory surfaces like fume cupboards, work tops or floors?

Efficient decontamination of actinides like americium, uranium, neptunium and plutonium from laboratory surfaces. The decontamination should be easy in the sense that it could be performed on a...

03 June 2020 8,707 5 View

What is the best way to organize your MSD V-PLEX Human Proinflammatory Panel 1 raw data for analysis?

Hello People, does anyone have experience with the raw data analyses of the MSD V-PLEX Human Proinflammatory Panel 1? A friend of mine ran 4 plates and struggles with organizing the raw data for...

01 April 2020 3,566 2 View

How to do a non-linear regression analysis with multiple variables and a strong confounding variable?

Hi, I did outgrowth assays on spiral ganglion neurons from the inner ear with each supplemented by different concentrations of two neurotrophins. For each explant I did an image analysis and...

29 March 2020 8,703 7 View

Would somebody have suggestions for bioinformatic tools that predict changes in enzymatic (e.g. kinase) function based on changes in AA sequence?

Prediction of enzymatic activity based on common genetic variation.

19 February 2020 6,582 2 View

What are minimum and maximum values for ratios in Geopolmers (Si:Al, Na2O:H20, NaO:SiO)?

I am trying to perform a screening DoE to further study influence of a range of factors for example Metakaolin content on various performances like compression strength and to further optimise my...

23 January 2020 5,020 3 View

How to compare diagnostic efficacy of several methods in a within-subjects design?

Hi, I conducted a study on faking in personality tests, where the participants received two instructions (respond honestly vs. fake good) in a within-subjects design (oder was counter-balanced...

09 January 2020 5,182 3 View

Why do I get a different results (load-displacement-curve) when applying different loads in ABAQUS/Explicit?

I'm currently modelling a steel-concrete composite construction with headed studs in ABAQUS/Explicit and want to analyse the load-displacement behaviour of the headed stud. In the first case I...

18 December 2019 3,070 4 View

What amount of data is necessary to extract meaningful/significant Sequential patterns / rules in a sequential database?

10 March 2019 5,559 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View